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Protokolle

Übersicht

1.)      DNA Purification Techniques
2.)      DNA Transformation/Library Preparation
3.)      Southern/Northern Blotting
4.)      DNA Sequencing
5.)      Oligonucleotides
6.)      PCR and Related Methods
7.)      RNA Methods
8.)      Protein Electrophoresis
9.)      Protein Purification
10.)    Autoradiography Tips
11.)    Safety
12.)    Other References

 

1.)    DNA Purification Techniques

  • DNA purification (plasmids, cosmids and p1) using Diatomaceous Earth

  • DNA purification, plasmid mini-preps, diatomaceous earth

  • DNA purification, bacterial-genomic, triton extraction method

  • DNA purification, bacterial-genomic via Qiagen columns

  • DNA purification, bacterial genomic, specifically Gram positive bacteria

  • DNA isolation from low melting-temperature agarose

  • Low copy plasmid purification using Qiagen Midi columns

  • Plasmid "Miniprep" Isolation: tried and true

  • Single stranded template from pBluescript phagemid

    >> up <<

    2.)    DNA Transformation/Library Preparation

  • Transformation-Competent E. coli preparation

  • Cosmid packaging protocol

    >> up <<

    3.)    Southern/Northern Blotting

  • Southern blot-chemiluminescent detection, using Lumigen or Lumiphos (BM "Genius" system)

  • Southern blot-chemiluminescent detection, using CSPD (BM "Genius" system)

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    4.)    DNA Sequencing

  • Sequencing gel solutions

  • Sequencing gels: wedge gel performance without pouring wedge gels

    >> up <<

    5.)    Oligonucleotides

  • Oligonucleotide Visualization

  • Oligonucleotide calculator: determine Tm, MW, etc.; need Javascript

    >> up <<

    6.)    PCR and Related Methods

  • Core sample PCR: a method to re-PCR unique bands from products of mixed size

  • Cloning PCR-Generated DNA Fragments

  • Site-directed Mutagenesis using PCR

  • Reverse Transcriptase PCR

  • Error Rates for Thermal Resistant DNA Polymerases

    >> up <<

    7.)    RNA Methods

  • Solublization of RNA in Formamide

  • RNAse Protection Assay

  • The "Neverfail" Northern Blot Hybridization

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    8.)    Protein Electrophoresis

  • Tricine-polyacrylamide gels: for high resolution of small proteins

  • SDS-polyacrylamide gels: standard Laemmli protocol

  • Ethanol-based SDS-PAGE Coomassie Blue staining

  • Bench protocol for silver staining SDS-polyacrylamide gels

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    9.)    Protein Purification

  • Preparation of active proteins from inclusion bodies

    >> up <<

    10.)    Autoradiography Tips

  • Intensifying Screens -- their use explained

    >> up <<

    11.)    Safety

  • Generation of Phosgene from Chloroform

    >> up <<

    12.)    Other References

  • Standard Genetic Code

  • Molecular Biology Protocols

  • Einige Methoden der Molekularbiologie

  • Protocols Used in the Roe Laboratory

  • Links to other Molecular Biology Protocols and Resources

     

     >> back <<       >> up <<